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Hematoxylin Eosin He Staining Kit, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: UCNPs@Ce6-based Near-Infrared photodynamic therapy triggers mitophagy-related apoptosis in esophageal squamous cell carcinoma
Article Snippet: Yttrium chloride hexahydrate (YCl3•6H2O), ytterbium chloride hexahydrate (YbCl3•6H2O), erbium chloride hexahydrate (ErCl3•6H2O), oleic acid (OA), 1-octadecene (ODE), and sodium hydroxide were purchased from Aladdin Chemical Reagent Co., Ltd.; Ammonium fluoride (NH4F), cyclohexane, and dihydroporphyrin (Ce6) (Shanghai McLean Bio-Chemical Technology Co., Ltd.), chloroform (Xilong Chemical Co., Ltd.); Dipalmitoy lphosphatidyl ethanolaminepolyethylene glycol 2000 (DSPE-mPEG2000) (Aivito Pharmaceutical Technology Co., Ltd.; Penicillin-streptomycin mixture (PS) (100 U/mL), SDS-PAGE protein loading buffer, and 4% formaldehyde fixative (Solarbio, China); RPMI 1640, minimal essential medium (MEM), and fetal bovine serum (FBS) (Gibco BRL Co., Ltd); CCK-8 assay kit and 1,3- diphenylisobenzofuran (DPBF) (Shanghai MCE Company); 2′-7′- Dichlorodihydrofluorescein diacetate (DCFH-DA), calcein AM/PI cell viability and cytotoxicity assay kit, 4′,6-diamidino-2-phenylindole (DAPI), enhanced ATP assay kit, monodansylcadaverine (MDC) staining assay kit, and mitochondrial membrane potential assay kit (JC-1) (Biyuntian Biotechnology Co., Ltd.); 2% mouse red blood cell solution (Shanghai Yuanye Biotechnology Co., Ltd.); Antibodies including Cleaver Caspase3, LC3, Beclin1, Parkin, PINK1, MLKL, RIP3, Caspase1, GSDMD, CRT, HSP70, β-actin, horseradish peroxidase (HRP)-conjugated goat anti-mouse or anti-rabbit secondary antibody (Wuhan Sanying); Antibodies including Bax (R22708), Bcl2 (R10047) (Zenbio); Antibodies including p62, ρ-MLKL, ρ-RIP3, and Cleaver GSDMD-N (Abcam); Goat anti-rabbit secondary antibody conjugated to either Alexa Fluor 488 or Alexa Fluor 594 (Zhongshan Jinqiao); Interleukin IL-18, IL-1β, IL-6, TNF-α, HMGB1 enzyme-linked immunosorbent assay (ELISA) kits (Elabscience Biotechnology Co. China); Ready-to-use BCA protein content detection kit, Omni-EasyTM One-Step Stain-Free PAGE Gel preparation kit, Feike General-Purpose Chemiluminescence detection kit, Annexin V-FITC/PI apoptosis kit, Tris-Buffered Saline-Tween (TBST) buffer, Ice-Free Rapid Blotting Buffer Granules, Tris-Glycine SDS electrophoresis buffer, and Three-Color Pre-stained Protein Marker (Shanghai Yaenzyme Biotechnology Co., Ltd.); Hematoxylin-eosin (HE) staining kit, Dihydroethidium (DHE) and sodium citrate buffer (Jiangsu KeyGEN BioTECH Co., Ltd.).

CCK-8 Assay:

Article Title: UCNPs@Ce6-based Near-Infrared photodynamic therapy triggers mitophagy-related apoptosis in esophageal squamous cell carcinoma
Article Snippet: Yttrium chloride hexahydrate (YCl3•6H2O), ytterbium chloride hexahydrate (YbCl3•6H2O), erbium chloride hexahydrate (ErCl3•6H2O), oleic acid (OA), 1-octadecene (ODE), and sodium hydroxide were purchased from Aladdin Chemical Reagent Co., Ltd.; Ammonium fluoride (NH4F), cyclohexane, and dihydroporphyrin (Ce6) (Shanghai McLean Bio-Chemical Technology Co., Ltd.), chloroform (Xilong Chemical Co., Ltd.); Dipalmitoy lphosphatidyl ethanolaminepolyethylene glycol 2000 (DSPE-mPEG2000) (Aivito Pharmaceutical Technology Co., Ltd.; Penicillin-streptomycin mixture (PS) (100 U/mL), SDS-PAGE protein loading buffer, and 4% formaldehyde fixative (Solarbio, China); RPMI 1640, minimal essential medium (MEM), and fetal bovine serum (FBS) (Gibco BRL Co., Ltd); CCK-8 assay kit and 1,3- diphenylisobenzofuran (DPBF) (Shanghai MCE Company); 2′-7′- Dichlorodihydrofluorescein diacetate (DCFH-DA), calcein AM/PI cell viability and cytotoxicity assay kit, 4′,6-diamidino-2-phenylindole (DAPI), enhanced ATP assay kit, monodansylcadaverine (MDC) staining assay kit, and mitochondrial membrane potential assay kit (JC-1) (Biyuntian Biotechnology Co., Ltd.); 2% mouse red blood cell solution (Shanghai Yuanye Biotechnology Co., Ltd.); Antibodies including Cleaver Caspase3, LC3, Beclin1, Parkin, PINK1, MLKL, RIP3, Caspase1, GSDMD, CRT, HSP70, β-actin, horseradish peroxidase (HRP)-conjugated goat anti-mouse or anti-rabbit secondary antibody (Wuhan Sanying); Antibodies including Bax (R22708), Bcl2 (R10047) (Zenbio); Antibodies including p62, ρ-MLKL, ρ-RIP3, and Cleaver GSDMD-N (Abcam); Goat anti-rabbit secondary antibody conjugated to either Alexa Fluor 488 or Alexa Fluor 594 (Zhongshan Jinqiao); Interleukin IL-18, IL-1β, IL-6, TNF-α, HMGB1 enzyme-linked immunosorbent assay (ELISA) kits (Elabscience Biotechnology Co. China); Ready-to-use BCA protein content detection kit, Omni-EasyTM One-Step Stain-Free PAGE Gel preparation kit, Feike General-Purpose Chemiluminescence detection kit, Annexin V-FITC/PI apoptosis kit, Tris-Buffered Saline-Tween (TBST) buffer, Ice-Free Rapid Blotting Buffer Granules, Tris-Glycine SDS electrophoresis buffer, and Three-Color Pre-stained Protein Marker (Shanghai Yaenzyme Biotechnology Co., Ltd.); Hematoxylin-eosin (HE) staining kit, Dihydroethidium (DHE) and sodium citrate buffer (Jiangsu KeyGEN BioTECH Co., Ltd.).

Cytotoxicity Assay:

Article Title: UCNPs@Ce6-based Near-Infrared photodynamic therapy triggers mitophagy-related apoptosis in esophageal squamous cell carcinoma
Article Snippet: Yttrium chloride hexahydrate (YCl3•6H2O), ytterbium chloride hexahydrate (YbCl3•6H2O), erbium chloride hexahydrate (ErCl3•6H2O), oleic acid (OA), 1-octadecene (ODE), and sodium hydroxide were purchased from Aladdin Chemical Reagent Co., Ltd.; Ammonium fluoride (NH4F), cyclohexane, and dihydroporphyrin (Ce6) (Shanghai McLean Bio-Chemical Technology Co., Ltd.), chloroform (Xilong Chemical Co., Ltd.); Dipalmitoy lphosphatidyl ethanolaminepolyethylene glycol 2000 (DSPE-mPEG2000) (Aivito Pharmaceutical Technology Co., Ltd.; Penicillin-streptomycin mixture (PS) (100 U/mL), SDS-PAGE protein loading buffer, and 4% formaldehyde fixative (Solarbio, China); RPMI 1640, minimal essential medium (MEM), and fetal bovine serum (FBS) (Gibco BRL Co., Ltd); CCK-8 assay kit and 1,3- diphenylisobenzofuran (DPBF) (Shanghai MCE Company); 2′-7′- Dichlorodihydrofluorescein diacetate (DCFH-DA), calcein AM/PI cell viability and cytotoxicity assay kit, 4′,6-diamidino-2-phenylindole (DAPI), enhanced ATP assay kit, monodansylcadaverine (MDC) staining assay kit, and mitochondrial membrane potential assay kit (JC-1) (Biyuntian Biotechnology Co., Ltd.); 2% mouse red blood cell solution (Shanghai Yuanye Biotechnology Co., Ltd.); Antibodies including Cleaver Caspase3, LC3, Beclin1, Parkin, PINK1, MLKL, RIP3, Caspase1, GSDMD, CRT, HSP70, β-actin, horseradish peroxidase (HRP)-conjugated goat anti-mouse or anti-rabbit secondary antibody (Wuhan Sanying); Antibodies including Bax (R22708), Bcl2 (R10047) (Zenbio); Antibodies including p62, ρ-MLKL, ρ-RIP3, and Cleaver GSDMD-N (Abcam); Goat anti-rabbit secondary antibody conjugated to either Alexa Fluor 488 or Alexa Fluor 594 (Zhongshan Jinqiao); Interleukin IL-18, IL-1β, IL-6, TNF-α, HMGB1 enzyme-linked immunosorbent assay (ELISA) kits (Elabscience Biotechnology Co. China); Ready-to-use BCA protein content detection kit, Omni-EasyTM One-Step Stain-Free PAGE Gel preparation kit, Feike General-Purpose Chemiluminescence detection kit, Annexin V-FITC/PI apoptosis kit, Tris-Buffered Saline-Tween (TBST) buffer, Ice-Free Rapid Blotting Buffer Granules, Tris-Glycine SDS electrophoresis buffer, and Three-Color Pre-stained Protein Marker (Shanghai Yaenzyme Biotechnology Co., Ltd.); Hematoxylin-eosin (HE) staining kit, Dihydroethidium (DHE) and sodium citrate buffer (Jiangsu KeyGEN BioTECH Co., Ltd.).

ATP Assay:

Article Title: UCNPs@Ce6-based Near-Infrared photodynamic therapy triggers mitophagy-related apoptosis in esophageal squamous cell carcinoma
Article Snippet: Yttrium chloride hexahydrate (YCl3•6H2O), ytterbium chloride hexahydrate (YbCl3•6H2O), erbium chloride hexahydrate (ErCl3•6H2O), oleic acid (OA), 1-octadecene (ODE), and sodium hydroxide were purchased from Aladdin Chemical Reagent Co., Ltd.; Ammonium fluoride (NH4F), cyclohexane, and dihydroporphyrin (Ce6) (Shanghai McLean Bio-Chemical Technology Co., Ltd.), chloroform (Xilong Chemical Co., Ltd.); Dipalmitoy lphosphatidyl ethanolaminepolyethylene glycol 2000 (DSPE-mPEG2000) (Aivito Pharmaceutical Technology Co., Ltd.; Penicillin-streptomycin mixture (PS) (100 U/mL), SDS-PAGE protein loading buffer, and 4% formaldehyde fixative (Solarbio, China); RPMI 1640, minimal essential medium (MEM), and fetal bovine serum (FBS) (Gibco BRL Co., Ltd); CCK-8 assay kit and 1,3- diphenylisobenzofuran (DPBF) (Shanghai MCE Company); 2′-7′- Dichlorodihydrofluorescein diacetate (DCFH-DA), calcein AM/PI cell viability and cytotoxicity assay kit, 4′,6-diamidino-2-phenylindole (DAPI), enhanced ATP assay kit, monodansylcadaverine (MDC) staining assay kit, and mitochondrial membrane potential assay kit (JC-1) (Biyuntian Biotechnology Co., Ltd.); 2% mouse red blood cell solution (Shanghai Yuanye Biotechnology Co., Ltd.); Antibodies including Cleaver Caspase3, LC3, Beclin1, Parkin, PINK1, MLKL, RIP3, Caspase1, GSDMD, CRT, HSP70, β-actin, horseradish peroxidase (HRP)-conjugated goat anti-mouse or anti-rabbit secondary antibody (Wuhan Sanying); Antibodies including Bax (R22708), Bcl2 (R10047) (Zenbio); Antibodies including p62, ρ-MLKL, ρ-RIP3, and Cleaver GSDMD-N (Abcam); Goat anti-rabbit secondary antibody conjugated to either Alexa Fluor 488 or Alexa Fluor 594 (Zhongshan Jinqiao); Interleukin IL-18, IL-1β, IL-6, TNF-α, HMGB1 enzyme-linked immunosorbent assay (ELISA) kits (Elabscience Biotechnology Co. China); Ready-to-use BCA protein content detection kit, Omni-EasyTM One-Step Stain-Free PAGE Gel preparation kit, Feike General-Purpose Chemiluminescence detection kit, Annexin V-FITC/PI apoptosis kit, Tris-Buffered Saline-Tween (TBST) buffer, Ice-Free Rapid Blotting Buffer Granules, Tris-Glycine SDS electrophoresis buffer, and Three-Color Pre-stained Protein Marker (Shanghai Yaenzyme Biotechnology Co., Ltd.); Hematoxylin-eosin (HE) staining kit, Dihydroethidium (DHE) and sodium citrate buffer (Jiangsu KeyGEN BioTECH Co., Ltd.).

Staining:

Article Title: UCNPs@Ce6-based Near-Infrared photodynamic therapy triggers mitophagy-related apoptosis in esophageal squamous cell carcinoma
Article Snippet: Yttrium chloride hexahydrate (YCl3•6H2O), ytterbium chloride hexahydrate (YbCl3•6H2O), erbium chloride hexahydrate (ErCl3•6H2O), oleic acid (OA), 1-octadecene (ODE), and sodium hydroxide were purchased from Aladdin Chemical Reagent Co., Ltd.; Ammonium fluoride (NH4F), cyclohexane, and dihydroporphyrin (Ce6) (Shanghai McLean Bio-Chemical Technology Co., Ltd.), chloroform (Xilong Chemical Co., Ltd.); Dipalmitoy lphosphatidyl ethanolaminepolyethylene glycol 2000 (DSPE-mPEG2000) (Aivito Pharmaceutical Technology Co., Ltd.; Penicillin-streptomycin mixture (PS) (100 U/mL), SDS-PAGE protein loading buffer, and 4% formaldehyde fixative (Solarbio, China); RPMI 1640, minimal essential medium (MEM), and fetal bovine serum (FBS) (Gibco BRL Co., Ltd); CCK-8 assay kit and 1,3- diphenylisobenzofuran (DPBF) (Shanghai MCE Company); 2′-7′- Dichlorodihydrofluorescein diacetate (DCFH-DA), calcein AM/PI cell viability and cytotoxicity assay kit, 4′,6-diamidino-2-phenylindole (DAPI), enhanced ATP assay kit, monodansylcadaverine (MDC) staining assay kit, and mitochondrial membrane potential assay kit (JC-1) (Biyuntian Biotechnology Co., Ltd.); 2% mouse red blood cell solution (Shanghai Yuanye Biotechnology Co., Ltd.); Antibodies including Cleaver Caspase3, LC3, Beclin1, Parkin, PINK1, MLKL, RIP3, Caspase1, GSDMD, CRT, HSP70, β-actin, horseradish peroxidase (HRP)-conjugated goat anti-mouse or anti-rabbit secondary antibody (Wuhan Sanying); Antibodies including Bax (R22708), Bcl2 (R10047) (Zenbio); Antibodies including p62, ρ-MLKL, ρ-RIP3, and Cleaver GSDMD-N (Abcam); Goat anti-rabbit secondary antibody conjugated to either Alexa Fluor 488 or Alexa Fluor 594 (Zhongshan Jinqiao); Interleukin IL-18, IL-1β, IL-6, TNF-α, HMGB1 enzyme-linked immunosorbent assay (ELISA) kits (Elabscience Biotechnology Co. China); Ready-to-use BCA protein content detection kit, Omni-EasyTM One-Step Stain-Free PAGE Gel preparation kit, Feike General-Purpose Chemiluminescence detection kit, Annexin V-FITC/PI apoptosis kit, Tris-Buffered Saline-Tween (TBST) buffer, Ice-Free Rapid Blotting Buffer Granules, Tris-Glycine SDS electrophoresis buffer, and Three-Color Pre-stained Protein Marker (Shanghai Yaenzyme Biotechnology Co., Ltd.); Hematoxylin-eosin (HE) staining kit, Dihydroethidium (DHE) and sodium citrate buffer (Jiangsu KeyGEN BioTECH Co., Ltd.).

Membrane:

Article Title: UCNPs@Ce6-based Near-Infrared photodynamic therapy triggers mitophagy-related apoptosis in esophageal squamous cell carcinoma
Article Snippet: Yttrium chloride hexahydrate (YCl3•6H2O), ytterbium chloride hexahydrate (YbCl3•6H2O), erbium chloride hexahydrate (ErCl3•6H2O), oleic acid (OA), 1-octadecene (ODE), and sodium hydroxide were purchased from Aladdin Chemical Reagent Co., Ltd.; Ammonium fluoride (NH4F), cyclohexane, and dihydroporphyrin (Ce6) (Shanghai McLean Bio-Chemical Technology Co., Ltd.), chloroform (Xilong Chemical Co., Ltd.); Dipalmitoy lphosphatidyl ethanolaminepolyethylene glycol 2000 (DSPE-mPEG2000) (Aivito Pharmaceutical Technology Co., Ltd.; Penicillin-streptomycin mixture (PS) (100 U/mL), SDS-PAGE protein loading buffer, and 4% formaldehyde fixative (Solarbio, China); RPMI 1640, minimal essential medium (MEM), and fetal bovine serum (FBS) (Gibco BRL Co., Ltd); CCK-8 assay kit and 1,3- diphenylisobenzofuran (DPBF) (Shanghai MCE Company); 2′-7′- Dichlorodihydrofluorescein diacetate (DCFH-DA), calcein AM/PI cell viability and cytotoxicity assay kit, 4′,6-diamidino-2-phenylindole (DAPI), enhanced ATP assay kit, monodansylcadaverine (MDC) staining assay kit, and mitochondrial membrane potential assay kit (JC-1) (Biyuntian Biotechnology Co., Ltd.); 2% mouse red blood cell solution (Shanghai Yuanye Biotechnology Co., Ltd.); Antibodies including Cleaver Caspase3, LC3, Beclin1, Parkin, PINK1, MLKL, RIP3, Caspase1, GSDMD, CRT, HSP70, β-actin, horseradish peroxidase (HRP)-conjugated goat anti-mouse or anti-rabbit secondary antibody (Wuhan Sanying); Antibodies including Bax (R22708), Bcl2 (R10047) (Zenbio); Antibodies including p62, ρ-MLKL, ρ-RIP3, and Cleaver GSDMD-N (Abcam); Goat anti-rabbit secondary antibody conjugated to either Alexa Fluor 488 or Alexa Fluor 594 (Zhongshan Jinqiao); Interleukin IL-18, IL-1β, IL-6, TNF-α, HMGB1 enzyme-linked immunosorbent assay (ELISA) kits (Elabscience Biotechnology Co. China); Ready-to-use BCA protein content detection kit, Omni-EasyTM One-Step Stain-Free PAGE Gel preparation kit, Feike General-Purpose Chemiluminescence detection kit, Annexin V-FITC/PI apoptosis kit, Tris-Buffered Saline-Tween (TBST) buffer, Ice-Free Rapid Blotting Buffer Granules, Tris-Glycine SDS electrophoresis buffer, and Three-Color Pre-stained Protein Marker (Shanghai Yaenzyme Biotechnology Co., Ltd.); Hematoxylin-eosin (HE) staining kit, Dihydroethidium (DHE) and sodium citrate buffer (Jiangsu KeyGEN BioTECH Co., Ltd.).

Enzyme-linked Immunosorbent Assay:

Article Title: UCNPs@Ce6-based Near-Infrared photodynamic therapy triggers mitophagy-related apoptosis in esophageal squamous cell carcinoma
Article Snippet: Yttrium chloride hexahydrate (YCl3•6H2O), ytterbium chloride hexahydrate (YbCl3•6H2O), erbium chloride hexahydrate (ErCl3•6H2O), oleic acid (OA), 1-octadecene (ODE), and sodium hydroxide were purchased from Aladdin Chemical Reagent Co., Ltd.; Ammonium fluoride (NH4F), cyclohexane, and dihydroporphyrin (Ce6) (Shanghai McLean Bio-Chemical Technology Co., Ltd.), chloroform (Xilong Chemical Co., Ltd.); Dipalmitoy lphosphatidyl ethanolaminepolyethylene glycol 2000 (DSPE-mPEG2000) (Aivito Pharmaceutical Technology Co., Ltd.; Penicillin-streptomycin mixture (PS) (100 U/mL), SDS-PAGE protein loading buffer, and 4% formaldehyde fixative (Solarbio, China); RPMI 1640, minimal essential medium (MEM), and fetal bovine serum (FBS) (Gibco BRL Co., Ltd); CCK-8 assay kit and 1,3- diphenylisobenzofuran (DPBF) (Shanghai MCE Company); 2′-7′- Dichlorodihydrofluorescein diacetate (DCFH-DA), calcein AM/PI cell viability and cytotoxicity assay kit, 4′,6-diamidino-2-phenylindole (DAPI), enhanced ATP assay kit, monodansylcadaverine (MDC) staining assay kit, and mitochondrial membrane potential assay kit (JC-1) (Biyuntian Biotechnology Co., Ltd.); 2% mouse red blood cell solution (Shanghai Yuanye Biotechnology Co., Ltd.); Antibodies including Cleaver Caspase3, LC3, Beclin1, Parkin, PINK1, MLKL, RIP3, Caspase1, GSDMD, CRT, HSP70, β-actin, horseradish peroxidase (HRP)-conjugated goat anti-mouse or anti-rabbit secondary antibody (Wuhan Sanying); Antibodies including Bax (R22708), Bcl2 (R10047) (Zenbio); Antibodies including p62, ρ-MLKL, ρ-RIP3, and Cleaver GSDMD-N (Abcam); Goat anti-rabbit secondary antibody conjugated to either Alexa Fluor 488 or Alexa Fluor 594 (Zhongshan Jinqiao); Interleukin IL-18, IL-1β, IL-6, TNF-α, HMGB1 enzyme-linked immunosorbent assay (ELISA) kits (Elabscience Biotechnology Co. China); Ready-to-use BCA protein content detection kit, Omni-EasyTM One-Step Stain-Free PAGE Gel preparation kit, Feike General-Purpose Chemiluminescence detection kit, Annexin V-FITC/PI apoptosis kit, Tris-Buffered Saline-Tween (TBST) buffer, Ice-Free Rapid Blotting Buffer Granules, Tris-Glycine SDS electrophoresis buffer, and Three-Color Pre-stained Protein Marker (Shanghai Yaenzyme Biotechnology Co., Ltd.); Hematoxylin-eosin (HE) staining kit, Dihydroethidium (DHE) and sodium citrate buffer (Jiangsu KeyGEN BioTECH Co., Ltd.).

Polyacrylamide Gel Electrophoresis:

Article Title: UCNPs@Ce6-based Near-Infrared photodynamic therapy triggers mitophagy-related apoptosis in esophageal squamous cell carcinoma
Article Snippet: Yttrium chloride hexahydrate (YCl3•6H2O), ytterbium chloride hexahydrate (YbCl3•6H2O), erbium chloride hexahydrate (ErCl3•6H2O), oleic acid (OA), 1-octadecene (ODE), and sodium hydroxide were purchased from Aladdin Chemical Reagent Co., Ltd.; Ammonium fluoride (NH4F), cyclohexane, and dihydroporphyrin (Ce6) (Shanghai McLean Bio-Chemical Technology Co., Ltd.), chloroform (Xilong Chemical Co., Ltd.); Dipalmitoy lphosphatidyl ethanolaminepolyethylene glycol 2000 (DSPE-mPEG2000) (Aivito Pharmaceutical Technology Co., Ltd.; Penicillin-streptomycin mixture (PS) (100 U/mL), SDS-PAGE protein loading buffer, and 4% formaldehyde fixative (Solarbio, China); RPMI 1640, minimal essential medium (MEM), and fetal bovine serum (FBS) (Gibco BRL Co., Ltd); CCK-8 assay kit and 1,3- diphenylisobenzofuran (DPBF) (Shanghai MCE Company); 2′-7′- Dichlorodihydrofluorescein diacetate (DCFH-DA), calcein AM/PI cell viability and cytotoxicity assay kit, 4′,6-diamidino-2-phenylindole (DAPI), enhanced ATP assay kit, monodansylcadaverine (MDC) staining assay kit, and mitochondrial membrane potential assay kit (JC-1) (Biyuntian Biotechnology Co., Ltd.); 2% mouse red blood cell solution (Shanghai Yuanye Biotechnology Co., Ltd.); Antibodies including Cleaver Caspase3, LC3, Beclin1, Parkin, PINK1, MLKL, RIP3, Caspase1, GSDMD, CRT, HSP70, β-actin, horseradish peroxidase (HRP)-conjugated goat anti-mouse or anti-rabbit secondary antibody (Wuhan Sanying); Antibodies including Bax (R22708), Bcl2 (R10047) (Zenbio); Antibodies including p62, ρ-MLKL, ρ-RIP3, and Cleaver GSDMD-N (Abcam); Goat anti-rabbit secondary antibody conjugated to either Alexa Fluor 488 or Alexa Fluor 594 (Zhongshan Jinqiao); Interleukin IL-18, IL-1β, IL-6, TNF-α, HMGB1 enzyme-linked immunosorbent assay (ELISA) kits (Elabscience Biotechnology Co. China); Ready-to-use BCA protein content detection kit, Omni-EasyTM One-Step Stain-Free PAGE Gel preparation kit, Feike General-Purpose Chemiluminescence detection kit, Annexin V-FITC/PI apoptosis kit, Tris-Buffered Saline-Tween (TBST) buffer, Ice-Free Rapid Blotting Buffer Granules, Tris-Glycine SDS electrophoresis buffer, and Three-Color Pre-stained Protein Marker (Shanghai Yaenzyme Biotechnology Co., Ltd.); Hematoxylin-eosin (HE) staining kit, Dihydroethidium (DHE) and sodium citrate buffer (Jiangsu KeyGEN BioTECH Co., Ltd.).

Saline:

Article Title: UCNPs@Ce6-based Near-Infrared photodynamic therapy triggers mitophagy-related apoptosis in esophageal squamous cell carcinoma
Article Snippet: Yttrium chloride hexahydrate (YCl3•6H2O), ytterbium chloride hexahydrate (YbCl3•6H2O), erbium chloride hexahydrate (ErCl3•6H2O), oleic acid (OA), 1-octadecene (ODE), and sodium hydroxide were purchased from Aladdin Chemical Reagent Co., Ltd.; Ammonium fluoride (NH4F), cyclohexane, and dihydroporphyrin (Ce6) (Shanghai McLean Bio-Chemical Technology Co., Ltd.), chloroform (Xilong Chemical Co., Ltd.); Dipalmitoy lphosphatidyl ethanolaminepolyethylene glycol 2000 (DSPE-mPEG2000) (Aivito Pharmaceutical Technology Co., Ltd.; Penicillin-streptomycin mixture (PS) (100 U/mL), SDS-PAGE protein loading buffer, and 4% formaldehyde fixative (Solarbio, China); RPMI 1640, minimal essential medium (MEM), and fetal bovine serum (FBS) (Gibco BRL Co., Ltd); CCK-8 assay kit and 1,3- diphenylisobenzofuran (DPBF) (Shanghai MCE Company); 2′-7′- Dichlorodihydrofluorescein diacetate (DCFH-DA), calcein AM/PI cell viability and cytotoxicity assay kit, 4′,6-diamidino-2-phenylindole (DAPI), enhanced ATP assay kit, monodansylcadaverine (MDC) staining assay kit, and mitochondrial membrane potential assay kit (JC-1) (Biyuntian Biotechnology Co., Ltd.); 2% mouse red blood cell solution (Shanghai Yuanye Biotechnology Co., Ltd.); Antibodies including Cleaver Caspase3, LC3, Beclin1, Parkin, PINK1, MLKL, RIP3, Caspase1, GSDMD, CRT, HSP70, β-actin, horseradish peroxidase (HRP)-conjugated goat anti-mouse or anti-rabbit secondary antibody (Wuhan Sanying); Antibodies including Bax (R22708), Bcl2 (R10047) (Zenbio); Antibodies including p62, ρ-MLKL, ρ-RIP3, and Cleaver GSDMD-N (Abcam); Goat anti-rabbit secondary antibody conjugated to either Alexa Fluor 488 or Alexa Fluor 594 (Zhongshan Jinqiao); Interleukin IL-18, IL-1β, IL-6, TNF-α, HMGB1 enzyme-linked immunosorbent assay (ELISA) kits (Elabscience Biotechnology Co. China); Ready-to-use BCA protein content detection kit, Omni-EasyTM One-Step Stain-Free PAGE Gel preparation kit, Feike General-Purpose Chemiluminescence detection kit, Annexin V-FITC/PI apoptosis kit, Tris-Buffered Saline-Tween (TBST) buffer, Ice-Free Rapid Blotting Buffer Granules, Tris-Glycine SDS electrophoresis buffer, and Three-Color Pre-stained Protein Marker (Shanghai Yaenzyme Biotechnology Co., Ltd.); Hematoxylin-eosin (HE) staining kit, Dihydroethidium (DHE) and sodium citrate buffer (Jiangsu KeyGEN BioTECH Co., Ltd.).

Electrophoresis:

Article Title: UCNPs@Ce6-based Near-Infrared photodynamic therapy triggers mitophagy-related apoptosis in esophageal squamous cell carcinoma
Article Snippet: Yttrium chloride hexahydrate (YCl3•6H2O), ytterbium chloride hexahydrate (YbCl3•6H2O), erbium chloride hexahydrate (ErCl3•6H2O), oleic acid (OA), 1-octadecene (ODE), and sodium hydroxide were purchased from Aladdin Chemical Reagent Co., Ltd.; Ammonium fluoride (NH4F), cyclohexane, and dihydroporphyrin (Ce6) (Shanghai McLean Bio-Chemical Technology Co., Ltd.), chloroform (Xilong Chemical Co., Ltd.); Dipalmitoy lphosphatidyl ethanolaminepolyethylene glycol 2000 (DSPE-mPEG2000) (Aivito Pharmaceutical Technology Co., Ltd.; Penicillin-streptomycin mixture (PS) (100 U/mL), SDS-PAGE protein loading buffer, and 4% formaldehyde fixative (Solarbio, China); RPMI 1640, minimal essential medium (MEM), and fetal bovine serum (FBS) (Gibco BRL Co., Ltd); CCK-8 assay kit and 1,3- diphenylisobenzofuran (DPBF) (Shanghai MCE Company); 2′-7′- Dichlorodihydrofluorescein diacetate (DCFH-DA), calcein AM/PI cell viability and cytotoxicity assay kit, 4′,6-diamidino-2-phenylindole (DAPI), enhanced ATP assay kit, monodansylcadaverine (MDC) staining assay kit, and mitochondrial membrane potential assay kit (JC-1) (Biyuntian Biotechnology Co., Ltd.); 2% mouse red blood cell solution (Shanghai Yuanye Biotechnology Co., Ltd.); Antibodies including Cleaver Caspase3, LC3, Beclin1, Parkin, PINK1, MLKL, RIP3, Caspase1, GSDMD, CRT, HSP70, β-actin, horseradish peroxidase (HRP)-conjugated goat anti-mouse or anti-rabbit secondary antibody (Wuhan Sanying); Antibodies including Bax (R22708), Bcl2 (R10047) (Zenbio); Antibodies including p62, ρ-MLKL, ρ-RIP3, and Cleaver GSDMD-N (Abcam); Goat anti-rabbit secondary antibody conjugated to either Alexa Fluor 488 or Alexa Fluor 594 (Zhongshan Jinqiao); Interleukin IL-18, IL-1β, IL-6, TNF-α, HMGB1 enzyme-linked immunosorbent assay (ELISA) kits (Elabscience Biotechnology Co. China); Ready-to-use BCA protein content detection kit, Omni-EasyTM One-Step Stain-Free PAGE Gel preparation kit, Feike General-Purpose Chemiluminescence detection kit, Annexin V-FITC/PI apoptosis kit, Tris-Buffered Saline-Tween (TBST) buffer, Ice-Free Rapid Blotting Buffer Granules, Tris-Glycine SDS electrophoresis buffer, and Three-Color Pre-stained Protein Marker (Shanghai Yaenzyme Biotechnology Co., Ltd.); Hematoxylin-eosin (HE) staining kit, Dihydroethidium (DHE) and sodium citrate buffer (Jiangsu KeyGEN BioTECH Co., Ltd.).

Marker:

Article Title: UCNPs@Ce6-based Near-Infrared photodynamic therapy triggers mitophagy-related apoptosis in esophageal squamous cell carcinoma
Article Snippet: Yttrium chloride hexahydrate (YCl3•6H2O), ytterbium chloride hexahydrate (YbCl3•6H2O), erbium chloride hexahydrate (ErCl3•6H2O), oleic acid (OA), 1-octadecene (ODE), and sodium hydroxide were purchased from Aladdin Chemical Reagent Co., Ltd.; Ammonium fluoride (NH4F), cyclohexane, and dihydroporphyrin (Ce6) (Shanghai McLean Bio-Chemical Technology Co., Ltd.), chloroform (Xilong Chemical Co., Ltd.); Dipalmitoy lphosphatidyl ethanolaminepolyethylene glycol 2000 (DSPE-mPEG2000) (Aivito Pharmaceutical Technology Co., Ltd.; Penicillin-streptomycin mixture (PS) (100 U/mL), SDS-PAGE protein loading buffer, and 4% formaldehyde fixative (Solarbio, China); RPMI 1640, minimal essential medium (MEM), and fetal bovine serum (FBS) (Gibco BRL Co., Ltd); CCK-8 assay kit and 1,3- diphenylisobenzofuran (DPBF) (Shanghai MCE Company); 2′-7′- Dichlorodihydrofluorescein diacetate (DCFH-DA), calcein AM/PI cell viability and cytotoxicity assay kit, 4′,6-diamidino-2-phenylindole (DAPI), enhanced ATP assay kit, monodansylcadaverine (MDC) staining assay kit, and mitochondrial membrane potential assay kit (JC-1) (Biyuntian Biotechnology Co., Ltd.); 2% mouse red blood cell solution (Shanghai Yuanye Biotechnology Co., Ltd.); Antibodies including Cleaver Caspase3, LC3, Beclin1, Parkin, PINK1, MLKL, RIP3, Caspase1, GSDMD, CRT, HSP70, β-actin, horseradish peroxidase (HRP)-conjugated goat anti-mouse or anti-rabbit secondary antibody (Wuhan Sanying); Antibodies including Bax (R22708), Bcl2 (R10047) (Zenbio); Antibodies including p62, ρ-MLKL, ρ-RIP3, and Cleaver GSDMD-N (Abcam); Goat anti-rabbit secondary antibody conjugated to either Alexa Fluor 488 or Alexa Fluor 594 (Zhongshan Jinqiao); Interleukin IL-18, IL-1β, IL-6, TNF-α, HMGB1 enzyme-linked immunosorbent assay (ELISA) kits (Elabscience Biotechnology Co. China); Ready-to-use BCA protein content detection kit, Omni-EasyTM One-Step Stain-Free PAGE Gel preparation kit, Feike General-Purpose Chemiluminescence detection kit, Annexin V-FITC/PI apoptosis kit, Tris-Buffered Saline-Tween (TBST) buffer, Ice-Free Rapid Blotting Buffer Granules, Tris-Glycine SDS electrophoresis buffer, and Three-Color Pre-stained Protein Marker (Shanghai Yaenzyme Biotechnology Co., Ltd.); Hematoxylin-eosin (HE) staining kit, Dihydroethidium (DHE) and sodium citrate buffer (Jiangsu KeyGEN BioTECH Co., Ltd.).



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Anti-PD-1 therapy improves the histopathology of lung and tumor tissue but induces myocarditis in wild-type mice. (A) Representative photographs of the lungs containing the tumors. HE staining was performed to examine the histopathology of (B) tumor (black arrow, nucleus; red arrow, inflammatory cell infiltration), (C) lung (arrow, interstitial congestion) and (D) heart tissue (black arrow, intercellular space; red arrow, inflammatory cell infiltration). (E) Masson's trichrome staining was performed to examine the histopathology of heart tissue. Arrow, collagen fiber. Scale bar, 50 µm. * P<0.05 vs. control, ### P<0.001 vs. LLC. HE, hematoxylin-eosin; LLC, Lewis lung carcinoma.

Journal: Biomedical Reports

Article Title: Preliminary exploration of the role of CD8 + T cells in anti-PD-1 antibody-induced myocarditis in C57BL/6 mice with Lewis lung carcinoma

doi: 10.3892/br.2026.2129

Figure Lengend Snippet: Anti-PD-1 therapy improves the histopathology of lung and tumor tissue but induces myocarditis in wild-type mice. (A) Representative photographs of the lungs containing the tumors. HE staining was performed to examine the histopathology of (B) tumor (black arrow, nucleus; red arrow, inflammatory cell infiltration), (C) lung (arrow, interstitial congestion) and (D) heart tissue (black arrow, intercellular space; red arrow, inflammatory cell infiltration). (E) Masson's trichrome staining was performed to examine the histopathology of heart tissue. Arrow, collagen fiber. Scale bar, 50 µm. * P<0.05 vs. control, ### P<0.001 vs. LLC. HE, hematoxylin-eosin; LLC, Lewis lung carcinoma.

Article Snippet: Masson's trichrome (cat. no. C0189S) and hematoxylin & eosin (HE) staining kit (cat. no. C0105S) were purchased from Beyotime Institute of Biotechnology.

Techniques: Histopathology, Staining, Control

Effects of anti-PD-1 therapy on the histopathology of tumor, lung and heart tissue in CD8 -/- mice. (A) Representative photographs of the lungs containing the tumors. HE staining was performed to examine the histopathology of (B) tumor (black arrow, nucleus; red arrow, inflammatory cell infiltration), (C) lung (arrow, interstitial congestion) and (D) heart tissue (black arrow, intercellular space; red arrow, inflammatory cell infiltration). (E) Masson's trichrome staining was performed to examine the histopathology of heart tissue. Arrow, collagen fiber. Scale bar, 50 µm. *** P<0.001. HE, hematoxylin-eosin; LLC, Lewis lung carcinoma; ns, not significant.

Journal: Biomedical Reports

Article Title: Preliminary exploration of the role of CD8 + T cells in anti-PD-1 antibody-induced myocarditis in C57BL/6 mice with Lewis lung carcinoma

doi: 10.3892/br.2026.2129

Figure Lengend Snippet: Effects of anti-PD-1 therapy on the histopathology of tumor, lung and heart tissue in CD8 -/- mice. (A) Representative photographs of the lungs containing the tumors. HE staining was performed to examine the histopathology of (B) tumor (black arrow, nucleus; red arrow, inflammatory cell infiltration), (C) lung (arrow, interstitial congestion) and (D) heart tissue (black arrow, intercellular space; red arrow, inflammatory cell infiltration). (E) Masson's trichrome staining was performed to examine the histopathology of heart tissue. Arrow, collagen fiber. Scale bar, 50 µm. *** P<0.001. HE, hematoxylin-eosin; LLC, Lewis lung carcinoma; ns, not significant.

Article Snippet: Masson's trichrome (cat. no. C0189S) and hematoxylin & eosin (HE) staining kit (cat. no. C0105S) were purchased from Beyotime Institute of Biotechnology.

Techniques: Histopathology, Staining